目的：通过光学相干断层扫描血管造影，对小鼠角膜上皮和角膜全层厚度进行检测并分析埃罗替尼对其的影响。

方法：随机均分20只小鼠为埃罗替尼组和PBS组，制备埃罗替尼滴眼液，分别应用埃罗替尼滴眼液和PBS，于每日8：00、12：00、16：00和20：00对2组小鼠点眼。在点眼前、点眼后1、2、3wk应用OCTA测量角膜17个区域的上皮和角膜全层厚度。

结果：点眼前埃罗替尼组与PBS组各区域角膜上皮和角膜全层厚度比较无差异(均P>0.05)； 点眼后第2wk，埃罗替尼组小鼠角膜上皮和角膜全层的17个区域中M、T5、IT5、I5、IN5、N5、T6、IT6、I6、IN6、N6区域与PBS组相比均明显增厚(均P<0.05)。第3wk，埃罗替尼组小鼠角膜上皮和角膜全层厚度的17个区域与PBS组相比均明显增厚(均P<0.05)； 埃罗替尼组和PBS组经处理2、3wk后，各组间角膜上皮厚度和角膜全层厚度平均值相比有差异(均P<0.05)； 通过角膜上皮和角膜全层厚度平均值变化趋势分析，埃罗替尼组与PBS组均有差异(均P<0.05)。

结论：利用OCTA可以发现埃罗替尼具有使角膜上皮和角膜全层增厚的作用，且随着应用次数的增多，其效果越明显。

METHODS: Totally 20 mice were randomly divided into experimental group and PBS group. Erlotinib eye drops was prepared. Erlotinib eye drops and PBS were applied to the two groups of mice at 8, 12, 16 and 20 o'clock each day. OCTA was used to measure the 17 regions of the epithelium and corneal thickness at 1wk, 2wk and 3wk before and after the eye droppings.

RESULTS: There was no significant difference in the thickness of corneal epithelium and cornea between the experimental group and PBS group(all P>0.05). Two weeks after eye dropping, the areas of M, T5, IT5, I5, IN5, N5, T6, IT6, IN6 and N6 in the epithelium and corneal of experimental group were significantly thicker than those of PBS group(all P<0.05). In the third week, 17 areas of epithelium and cornea in experimental group were significantly thicker than those of PBS group(all P<0.05). After treatment with 2 and 3wk in erotinib group and PBS group, there were differences in the average corneal epithelial thickness and the total corneal thickness between each group(all P<0.05). According to the trend analysis of the average change of corneal epithelium and corneal thickness, there were differences between the erotinib group and the PBS group(all P<0.05).

CONCLUSION: Using OCTA, it can be found that ellotini has the effect of thickening corneal epithelium and cornea, and the effect is more obvious with the increase of application times.