目的：评价地塞米松不同途径给药对2型糖尿病(T2DM)大鼠房水药物浓度和血糖的影响。

方法：将造模成功的T2DM大鼠随机分为实验组(注射地塞米松磷酸钠)和对照组(注射生理盐水)，每组根据给药途径不同随机分为球旁注射组、球后注射组、结膜下注射组三个亚组。注射0～24h采集大鼠尾静脉血经试纸法测定血糖浓度，注射0.5～24h，采用高效液相-质谱法测定大鼠双眼房水内地塞米松浓度。

结果：不同途径注射地塞米松磷酸钠或生理盐水对T2DM大鼠血糖浓度的影响无明显差异(P>0.05)。不同途径注射地塞米松磷酸钠后0.5～24h，实验组大鼠双眼房水地塞米松浓度峰值：结膜下注射组(957.34±3.60ng/mL)>球后注射组(859.60±3.56ng/mL)>球旁注射组(732.38±4.56ng/mL)。

结论：球旁、球后、结膜下注射地塞米松磷酸钠对T2DM大鼠血糖影响无明显差异，而结膜下注射操作简单，较球旁和球后注射可在短时间内达到较高的药物浓度。

METHODS: The T2DM rats successfully modeled were randomly divided into the experimental group(dexamethasone sodium phosphate injection)and the control group(normal saline injection). Each group was randomly divided into three subgroups: peribulbar injection group, post-bulbar injection group and subconjunctival injection group according to different drug delivery routes. Rat tail venous blood was collected 0-24h after injection and blood glucose concentration was determined by dipstick method. After injection, dexamethasone concentration in aqueous aqueous solution of both eyes was determined by HPLC at 0.5-24h.

RESULTS: Dexamethasone sodium phosphate or normal saline injection had no significant effect on blood glucose concentration in T2DM rats(P>0.05). After dexamethasone sodium phosphate was injected in different ways for 0.5-24h, rats in the experimental group received dexamethasone concentration peak under bulbar conjunctiva injection group(957.34±3.60ng/mL)>post-bulbar injection group(859.60±3.56ng/mL)>perbulbar injection group(732.38±4.56ng/mL).

CONCLUSION: Injection of dexamethasone sodium phosphate into bulbous injection, post-bulbar injection and subconjunctival injection had no significant effect on blood glucose of T2DM rats, while subconjunctival injection was simple and could reach higher drug concentration in a short time than that injected into bulbous injection and sub-bulbous injection.