目的：探讨沉默SIAH1基因对H₂O₂诱导的人晶状体上皮细胞凋亡的影响。

方法：将培养的人晶状体上皮细胞系HLE-B3分为正常组、H₂O₂组(用含400μmol/L H₂O₂的培养液培养)、H₂O₂+siR-SIAH1组(转染SIAH1干扰序列后用含400μmol/L H₂O₂培养液培养)和siR-NC组(转染阴性对照序列后用含400μmol/L H₂O₂培养液培养)，采用实时荧光定量PCR技术检测细胞中SIAH1基因表达，流式细胞术检测细胞凋亡率，Western blot法检测细胞中p38 MAPK、p-p38 MAPK、Bcl-2和Bax蛋白表达。

结果：H₂O₂组、siR-NC组和H₂O₂+siR-SIAH1组细胞中SIAH1 mRNA相对表达量均高于正常组，而H₂O₂+siR-SIAH1组细胞中SIAH1 mRNA相对表达量低于H₂O₂组和siR-NC组(均P<0.05)。H₂O₂组、siR-NC组和H₂O₂+siR-SIAH1组细胞凋亡率均高于正常组，而H₂O₂+siR-SIAH1组细胞凋亡率低于H₂O₂组和siR-NC组(均P<0.05)。H₂O₂组、siR-NC组和H₂O₂+siR-SIAH1组细胞中p38 MAPK和Bcl-2蛋白表达量低于正常组，而p-p38 MAPK和Bax蛋白表达量高于正常组，H₂O₂+siR-SIAH1组细胞中p38 MAPK和Bcl-2蛋白表达量高于H₂O₂组和siR-NC组，而p-p38 MAPK和Bax蛋白表达量低于H₂O₂组和siR-NC组(均P<0.05)。

结论：下调SIAH1基因表达可抑制H₂O₂诱导的人晶状体上皮细胞凋亡，其机制可能与抑制p38 MAPK信号通路活化有关。

METHODS: The human lens epithelial cell line HLE-B3 was cultured and divided into normal group, H₂O₂ group(cultured with medium containing 400μmol/L H₂O₂)and H₂O₂+siR-SIAH1 group(transfected with SIAH1 interference sequence, followed by cultured with medium containing 400μmol/L H₂O₂)and siR-NC group(transfected with negative control sequence, followed by cultured with medium containing 400μmol/L H₂O₂). The expression of SIAH1 gene in cells was detected by real-time fluorescent quantitative PCR. The apoptosis rate was detected by flow cytometry. The expressions of p38 MAPK, p-p38 MAPK, Bcl-2 and Bax proteins were detected by Western blot.

RESULTS: The relative expression levels of SIAH1 mRNA in the H₂O₂ group, siR-NC group and H₂O₂+siR-SIAH1 group were higher than that in the normal group(P<0.05). The relative expression level of SIAH1 mRNA in H₂O₂+siR-SIAH1 group was lower than those in the H₂O₂ group and siR-NC group(P<0.05). The apoptosis rates in the H₂O₂ group, siR-NC group and H₂O₂+siR-SIAH1 group were higher than that in the normal group(P<0.05). The apoptosis rate in the H₂O₂+siR-SIAH1 group was lower than those in the H₂O₂ group and siR-NC group(P<0.05). The expression levels of p38 MAPK and Bcl-2 proteins in the H₂O₂ group, siR-NC group and H₂O₂+siR-SIAH1 group were lower than those in the normal group, while the expression levels of p-p38 MAPK and Bax proteins were higher than those in the normal group(P<0.05). The expression levels of p38 MAPK and Bcl-2 proteins in the H₂O₂+siR-SIAH1 group were higher than those in the H₂O₂ group and siR-NC group, while the expression levels of p-p38 MAPK and Bax proteins were lower than those in the H₂O₂ group and siR-NC group(P<0.05).

CONCLUSION: Down-regulation the expression of SIAH1 gene could inhibit H₂O₂-induced apoptosis of human lens epithelial cell line HLE-B3, which might be related to inhibition of p38 MAPK signaling pathway activation.