目的：基于455～470nm面阵蓝光照射SD大鼠，观察大鼠视网膜及脉络膜组织结构变化并分析照射时间与组织结构变化之间的关系。

方法：选取6周龄健康SD雄性大鼠24只，随机分为正常对照组(n=6)和实验组(n=18)，正常对照组无任何干预正常喂养6wk； 实验组分为3个小组，每天分别予面阵蓝光发光器(455～470nm，391Lx)照射3、6、12h，连续照射6wk。光学显微镜下观察组织结构变化。

结果：正常对照组的大鼠眼底组织结构完整，细胞形态正常。随蓝光照射时间延长，各实验组大鼠脉络膜纤维结缔组织玻璃样改变，局部疏松水肿，小血管增生，色素层变薄，细胞稀疏，视细胞数逐渐减少，局部消失。3h实验组细胞核染色清晰，双极细胞层及节细胞层未见明确改变。6、12h实验组细胞核固缩，双极细胞层轻度增生，神经节细胞层局部形成胞突。

结论：视网膜的光感受器细胞随蓝光照射时间的延长出现明显变薄、萎缩、消失。色素上皮细胞损伤程度与蓝光照射时间无明显关系。

METHODS: Totally 24 healthy SD male rats of 6wk were selected and randomly divided into normal control group(n=6)and experimental group(n=18). The normal control group was fed for 6wk without any intervention. The experimental group was divided into three groups, which were exposed to the array blue light emitting apparatus(455nm-470nm, 391Lx)for 3, 6 and 12h each day for 6wk.

RESULTS: The fundus tissue structure was intact and the cell morphology was normal in the control group. With the extension of blue light irradiation time, the choroid fiber connective tissue of rats in all experimental groups presented hyaline changes, local loose edema, proliferation of small blood vessels, thinning of pigment layer, thinning of cells, gradual reduction of the number of visual cells, and local disappearance. At 3h, the nucleus staining of the experimental group was clear, and no definite changes were observed in the bipolar cell layer and ganglion cell layer. In the 6h and 12h groups, nucleus pyknosis was observed, bipolar cell layer was mildly proliferated, and local cytoplasm was formed in the ganglion cell layer.

CONCLUSION: The photoreceptor cells of retina become thinner, atrophy and disappear with the extension of blue light irradiation time. There was no significant relationship between the injury of pigment epithelial cells and the prolonged exposure time of blue light.