目的：检测常见泪腺上皮性肿瘤中类表皮生长因子结构域 7(EGFL7)、微血管密度(MVD)、Ki67的表达，探究EGFL7与MVD、Ki67之间的相互关系。

方法：应用免疫组化法，对10例正常泪腺组织(取自因炎性假瘤、Mikulicz病等良性泪腺肿瘤切除后，标本中的部分正常泪腺组织)、20例泪腺多形性腺瘤、12例多形性腺癌、14例腺样囊性癌组织中的EGFL7蛋白、Ki67进行检测，并应用CD34标记计数MVD。

结果：EGFL7主要在泪腺多形性腺癌、腺样囊性癌的肿瘤细胞的细胞浆中表达，免疫组化染色结果显示，在正常泪腺组织中EGFL7无阳性表达，泪腺多形性腺瘤组织中EGFL7的阳性率为5%(1/20)，泪腺多形性腺癌中EGFL7的阳性表达率83%(10/12)，泪腺腺样囊性癌中EGFL7的阳性表达率为86%(12/14)，两种恶性肿瘤中的表达率明显高于多形性腺瘤和正常泪腺组织(P<0.001)。CD34可使肿瘤微血管染色呈棕黄色的单个或成簇细胞群。在泪腺多形性腺癌(32.58±14.46)及腺样囊性癌(43.43±4.60)中CD34的表达明显高于多形性腺瘤(4.20±1.19)(P<0.001)； Ki67在具有增殖活性的细胞核呈棕褐色着色，在泪腺多形性腺癌(44.83±13.68)及腺样囊性癌(26.29±8.44)中Ki67的表达明显高于多形性腺瘤(2.80±3.14)及正常组织(0.40±0.70)(P<0.001)。在两种恶性肿瘤中EGFL7的表达分别与MVD、Ki67呈明显正相关(r_s=0.897，P<0.001； r_s=0.837，P<0.001)。

结论：EGFL7在泪腺上皮性肿瘤中的表达与MVD、Ki67呈正相关性，提示EGFL7不仅在泪腺上皮性肿瘤血管生成中起重要作用，而且参与泪腺上皮性肿瘤的增殖。

METHODS: A total of 46 paraffin-embedded specimens of common lacrimal gland epithelial tumors and other lacrimal gland tumor types, including 20 cases of lacrimal gland pleomorphic adenoma, 12 cases of pleomorphic adenocarcinoma, and 14 cases of adenoid cystic carcinoma, as well as ten normal lacrimal glands were analyzed for the expression of EGFL7 protein. For all specimens, the tumor microvascular networks were also labeled with anti-CD34 antibody and the tumor MVD was calculated. The proliferative activity of tumor cells containing Ki67.

RESULTS: EGFL7 protein was scored as positive with the presence of brown color in the cytoplasm, and was mainly observed in cells of lacrimal gland pleomorphic adenocarcinomas and adenoid cystic carcinomas. Immunohistochemical staining showed that EGFL7 was not expressed in normal lacrimal gland tissue. The rates of expression of EGFL7 in lacrimal gland pleomorphic adenomas, lacrimal gland pleomorphic adenocarcinomas, and lacrimal adenoid cystic carcinomas were 5%(1/20), 83%(10/12), and 86%(12/14), respectively. The EGFL7 expression in both malignant tumor types was significantly higher than that in pleomorphic adenomas and normal lacrimal gland tissues(P<0.001). CD34 staining colored the tumor microvascular network brown-yellow in single cells or clustered cell populations. The expression of CD34 in lacrimal gland pleomorphic adenocarcinomas(32.58±14.46)and adenoid cystic carcinomas(43.43±4.60)was significantly higher than that in pleomorphic adenomas(4.20±1.19)(P<0.001). Ki67 staining appeared as a brownish color in cell nuclei, indicating proliferative activity. The expression of Ki67 in lacrimal gland pleomorphic adenocarcinomas(44.83±13.68)and adenoid cystic carcinomas(26.29±8.44)was significantly higher than that in pleomorphic adenomas(2.80±3.14)and normal tissues(0.40±0.70)(P<0.001). Furthermore, the expression of EGFL7 protein was positively correlated with high MVD and Ki67 expression in lacrimal epithelial tumors(r_s=0.897, P<0.001; r_s=0.837, P<0.001).

CONCLUSION: The correlation of EGFL7 expression with high MVD and Ki67 expression suggests that high EGFL7 expression plays an important role in promoting tumor angiogenesis and tumor proliferation.