目的：观察G补缀FHA域血管生成因子1(AGGF1)在糖尿病视网膜组织及高糖条件下视网膜血管内皮细胞中的表达，以及AGGF1对视网膜血管内皮细胞增殖、迁移和管腔形成的影响。

方法：将C57BL/6J小鼠随机分为对照组和糖尿病视网膜病变(DR)模型组，采用免疫组化法检测视网膜组织中AGGF1的蛋白表达。将体外培养的恒河猴脉络膜视网膜血管内皮细胞(RF/6A细胞)随机分为对照组(低糖环境培养)和高糖组(培养基中加入25mmol/L D-葡萄糖)，采用免疫荧光法检测细胞中AGGF1的蛋白表达。然后将RF/6A细胞分为对照组和AGGF1处理组，分别采用CCK-8法、Transwell法和Matrigel检测细胞增殖、迁移及管腔形成。

结果：AGGF1蛋白在视网膜各层均有表达，在血管内皮细胞中也有明显表达，AGGF1在DR组视网膜中的表达(0.17±0.05)明显强于对照组(0.07±0.02)(P<0.05)。AGGF1蛋白在高糖组和对照组RF/6A细胞中均有表达，AGGF1在高糖下RF/6A细胞中的表达(0.63±0.10)明显强于对照组(0.40±0.03)(P<0.05)。处理12h，AGGF1组细胞增殖率(114.88%±0.84%)明显高于对照组(100.00%±2.17%)(P<0.05)； 处理24h，AGGF1组细胞增殖率(157.35%±1.89%)明显高于对照组(142.77%±0.50%)(P<0.05)； 处理48h，AGGF1组细胞增殖率(185.39%±1.90%)明显高于对照组(160.17%±1.33%)(P<0.05)。处理12h，AGGF1组细胞迁移数(127.00±7.00个)明显多于对照组(90.33±6.66个)(P<0.05)。处理12h，AGGF1组细胞管腔数(33.67±1.15个)明显多于对照组(15.33±3.51个)(P<0.05)； AGGF1组细胞分支总长度(8226.33±288.55μm)明显多于对照组(6463.33±938.01μm)(P<0.05)。

结论：糖尿病视网膜组织和高糖诱导的视网膜血管内皮细胞表达AGGF1蛋白明显增多，AGGF1可促进视网膜血管内皮细胞增殖、迁移和管腔形成，提示AGGF1可能参与了DR的视网膜新生血管形成。

METHODS: C57BL/6J mice were randomly divided into the control group and diabetic retinopathy(DR)model group. The cultured rhesus monkey choroido-retinal endothelial cells(RF/6A cells)were randomly divided into the control group(cultured in low-glucose environment)and the high-glucose group(cultured in medium with 25mmol/L D-glucose), and the AGGF1 protein expression in the cells was detected by immunofluorescence assay. RF/6A cells were then divided into the control group and AGGF1 treatment group, and cell proliferation, migration and tube formation was detected by CCK-8, Transwell and Matrigel, respectively.

RESULTS: AGGF1 protein was expressed in all layers of the retinas and in vascular endothelial cells. The expression of AGGF1 in the retinas of DR group(0.17±0.05)was significantly higher than that of the control group(0.07±0.02)(P<0.05). AGGF1 protein was expressed in RF/6A cells in both the high glucose group and the control group, and the expression of AGGF1 in RF/6A cells under high glucose was significantly higher(0.63±0.10)than that in the control group(0.40±0.03)(P<0.05). After 12h of treatment, the cell proliferation rate(114.88%±0.84%)in the AGGF1 group was significantly higher than that in the control group(100.00%±2.17%)(P<0.05). After 24h of treatment, the cell proliferation rate of the AGGF1 group(157.35%±1.89%)was significantly higher than that of the control group(142.77%±0.50%)(P<0.05). After 48h of treatment, the cell proliferation rate of the AGGF1 group(185.39%±1.90%)was significantly higher than that of the control group(160.17%±1.33%)(P<0.05). After 12h of treatment, the number of migrated cells(127.00±7.00)in the AGGF1 group was significantly higher than that in the control group(90.33±6.66)(P<0.05). After 12h of treatment, the number of tube formation(33.67±1.15)in the AGGF1 group was significantly higher than that in the control group(15.33±3.51)(P<0.05). The total tube length in AGGF1 group(8226.33±288.55)μm was significantly higher than that in the control group(6463.33±938.01)μm(P<0.05).

CONCLUSION: The expression of AGGF1 protein was significantly increased in diabetic retinal tissues and retinal vascular endothelial cells induced by high glucose. AGGF1 can promote the proliferation, migration and tube formation of retinal vascular endothelial cells, suggesting that AGGF1 may be involved in retinal neovascularization of DR.