目的：探索阿昔洛韦(ACV)对体外培养人眼Tenon囊成纤维细胞(HTFs)的增殖和凋亡的影响以及作用机制。

方法：将HTFs分为ACV处理组和空白组； CCK8检测不同浓度梯度下的细胞增殖速率，划痕实验检测HTFs迁移能力，流式细胞术检测HTFs凋亡以及细胞周期。

结果：与空白组相比，ACV处理组(终浓度分别为1.125、2.25、3.375、4.5mmol/L)HTFs增殖速率显著降低(P<0.05)，且呈浓度依赖性。4.5mmol/L ACV处理组划痕细胞迁移率显著降低(P<0.05)，细胞凋亡率显著增加(P=0.0005)，细胞周期G0/G1期峰值升高(P=0.0011)，S期下降(P=0.0006)，细胞周期被阻滞在G0/G1期。

结论：阿昔洛韦可以通过阻滞HTFs周期促进细胞凋亡，抑制HTFs的增殖和迁移。

METHODS: HTFs were divided into ACV treatment group and control group; CCK8 was used to detect cell proliferation rate under different concentration gradients, scratch assay was used to detect HTFs migration ability, and flow cytometry was used to detect HTFs apoptosis and cell cycle.

RESULTS: Compared with the control group, the proliferation rate of HTFs in the ACV-treated group(final concentrations were 1.125, 2.25, 3.375, 4.5mmol/L)was significantly reduced(P<0.05)and was concentration-dependent. The scratch closure rate in the ACV-treated group(final concentrations were 4.5mmol/L)was significantly reduced(P<0.05), the apoptosis rate was significantly increased(P=0.0005), the peak value of the cell cycle G0 / G1 increased(P=0.0011), and the S-phase decreased(P=0.0006). The cell cycle is blocked in the G0/G1 phase.

CONCLUSION: Acyclovir can promote cell apoptosis by blocking the cell cycle of HTFs, and inhibit the proliferation and migration of HTFs.