目的：探讨阻断短波可见光(400～450nm)后对健康人群泪膜稳定性的影响。

方法：前瞻性干预试验。2020-02/03选取在校大学生26名，其中男12名，女14名，平均年龄22.1±1.4岁。受试者在相邻两天的同一时间，在相同环境条件下观看视频1h，第一次无短波可见光阻断，第二次戴阻断短波可见光的近视或平光眼镜。分别记录观看视频前及两次观看视频后的双眼泪河弯曲面的曲率半径、泪液渗透压、泪膜破裂时间、角膜荧光素钠染色评分，并在试验结束后完成视觉感受调查问卷。

结果：泪河弯曲面的曲率半径、泪液渗透压、泪膜破裂时间、角膜荧光素钠染色评分在基础状态和短波可见未阻断时相比均有差异(t=4.50、5.72、4.437、3.245， 均P<0.05)，短波可见光阻断后与基础状态相比均无差异(t=1.972、1.993、1.921、1.641， 均P>0.05)，短波可见光阻断后与短波可见光未阻断时相比均有差异(t=2.825、3.771、2.610、3.028， 均P<0.05)。调查问卷结果显示：18名受试者(69%)表示短波可见光阻断与否均在观看视频后出现轻微眼睛干涩、疼痛、异物感、视疲劳等不适； 16名受试者(62%)表示短波可见光阻断后更舒适； 24名受试者(92%)表示愿意配戴阻断短波可见光的眼镜。

结论：短时间观看显示屏即会造成泪膜稳定性降低，阻断短波可见光可保护眼表泪膜的稳定性。

METHODS: In this prospective intervention experiment, 26 college students were selected, including 12 males and 14 females, aged 22.1±1.4 years, from February to March 2020. At the same time of two adjacent days, the subjects watched the video for 1h under the same environmental conditions. For the first time, there was no short wave visible light blocking, and for the second time, they wore short wave visible light blocking myopia or flat glasses. The subjects were examined the radius of curvature of lacrimal River curved surface, tear osmolality, tear film rupture time, corneal fluorescein sodium staining score, the basic state before watching the video and after watching the video respectively. And the questionnaire of visual perception was completed at the end of the experiment.

RESULTS: There were significant differences in the radius of curvature of lacrimal River curved surface, tear osmolality, tear film rupture time, corneal fluorescein sodium staining score between the basic state and the short wave visible unstopped state(t=4.50, 5.72, 4.437, 3.245, P<0.05). There was no significant difference between the short wave visible light blocking and the basic state(t=1.972, 1.993, 1.921, 1.641, P>0.05). There was also significant difference between the short wave visible light blocking and the short wave visible light unblocking(t=2.825, 3.771, 2.610, 3.028, P< 0.05). The results of the questionnaire showed that: eighteen subjects(69%)said that wearing the short wave visible light blocking glasses or without wearing short wave visible light blocking glasses had slight eye dryness, pain, foreign body sensation, visual fatigue and other discomfort after watching the video. Sixteen subjects(62%)said that they were more comfortable after blocking the short wave visible light. Twenty-four subjects(92%)said that they were willing to block the short wave visible light.

CONCLUSION: The stability of tear film could be reduced by watching the display screen for a short time. Blocking the short wave visible light could protect the stability of tear film of the ocular surface.