目的：从分子水平探讨全反式视黄酸(ATRA)诱导ARPE-19细胞的内质网应激反应(ERS)。

方法：应用免疫荧光法、实时定量-聚合酶链反应和蛋白印记法等方法，检测ATRA诱导ARPE-19细胞产生ERS过程中相关信号通路蛋白及mRNA表达水平的变化。

结果：检测结果显示，随着ATRA浓度的累积，ERS标记蛋白CHOP及BIP的蛋白及mRNA水平显著上升(P<0.001)； 下游信号通路中，PERK、EIF2α、ATF4、IRE1α及XBP1表达上调(P<0.001)，ATF6表达无变化(P>0.05)。

结论：过度累积的ATRA诱导ARPE-19细胞产生ERS，并激活其中PERK-EIF2α-ATF4及IRE1α-XBP1信号途径。

METHODS:Immunofluorescence, real-time quantitative polymerase chain reaction and Western blot were used to detect the protein and mRNA expression of related signal pathways during the process of endoplasmic reticulum stress response induced by ATRA in ARPE-19 cells.

RESULTS: With the accumulation of ATRA concentration, the protein and mRNA levels of endoplasmic reticulum stress response marker proteins chop and BiP were significantly increased(P<0.001); in the downstream signaling pathways, perk, eIF2 α, ATF4, IRE1 α and XBP1 were up-regulated(P<0.001), while the expression of ATF6 did not change(P>0.05).

CONCLUSION: Over accumulation of ATRA induces ERS in ARPE-19 cells and activates PERK-EIF2 α-ATF4 and IRE1 α-XBP1 signaling pathways