目的：探讨重组人生长激素(rHGH)对兔角膜上皮损伤早期的修复作用。

方法：选取32只健康雄性新西兰兔制作双眼角膜上皮损伤模型，随机选取1眼滴无菌生理盐水作为对照组，另1眼滴20nmol/L重组人生长激素作为rHGH组，分别于造模前、造模后24、48、72h采用荧光素钠染色法观察角膜上皮愈合情况，采用Cochet-Bonnet角膜知觉测量仪测量检查中央角膜敏感度，同时收集各时间点泪液检测炎症因子的表达。

结果：造模后48h对照组和rHGH组角膜上皮愈合率分别为62.52%±6.73%和79.62%±10.62%(P<0.05)，造模后72h分别为90.56%±9.57%和98.43%±3.65%(P<0.05)。造模后48h，对照组和rHGH组中央角膜敏感度分别为3.22±0.42、4.22±0.26cm(P<0.05)。造模后各时间点两组泪液TNF-α和IL-1α表达均较造模前增加，且对照组均高于rHGH组。造模后24、48h，两组泪液MMP-9表达均较造模前增加，且造模后48h对照组高于rHGH组(均P<0.05)。造模后24、48h两组泪液IL-21表达均较造模前增加(P<0.05)。造模后各时间点两组泪液IL-17α、Leptin表达与造模前相比及两组之间比较均无差异(P>0.05)。

结论：重组人生长激素有利于兔角膜上皮损伤早期修复。

METHODS:Thirty-two New Zealand rabbits were selected to establish the corneal epithelial defects models. One eye was treated with normal saline(NC group)and the other eye was treated with 20nmol/L rHGH(rHGH group)in a randomized double-blinded way. The corneal healing process was monitored by the corneal fluorescein staining scores at 0h, 24h, 48h, and 72h after the surgery. The central corneal sensitivity was detected by Cochet-Bonnet corneal esthesiometer and the concentrations of inflammatory mediators interlecukin-1α(IL-1α), interlecukin-17(IL-17), interlecukin-21(IL-21), Leptin, matrix metalloproteinase-9(MMP-9)and tumor necrosis factor-α(TNF-α)in collected tears were measured by multiplex antibody microarray.

RESULTS: The corneal epithelial healing rates of the NC group and rHGH group were(62.52±6.73)% and(79.62±10.62)%(P<0.05),(90.56±9.57)% and(98.43±3.65)%(P<0.05)at 48h and 72h postoperatively. The central corneal sensitivity of rHGH group(4.22±0.26)cm was better than that in NC group(3.22±0.42)cm at 48h after surgery(P<0.05). The expressions of TNF-α and IL-1α increased in both groups at each time point after operation, and the expressions in NC group were higher than those in the rHGH group. Both groups had higher MMP-9 concentrations in the tear fluid at 24 and 48h postoperation in comparison with the point before the operation. The MMP-9 expression in NC group was higher than that in the rHGH group at 48h postoperatively. The expressions of IL-21 in NC group were higher than those in the rHGH group at 24 and 48h postoperation in comparison with the point before the operation(P<0.05). No significant differences in tearIL-17 and Leptin were observed between groups before and after surgery(P>0.05).

CONCLUSION: Topical application of rHGH can accelerate the early stage of rabbit corneal epithelial wound healing in vivo.