方法：选取2014-02/2019-08在我院接受手术治疗的UM患者53例53眼，同期留取因外伤摘除眼球的正常葡萄膜组织34例34眼。采用免疫组织化学法检测组织中HMGA1蛋白表达。将体外培养的人UM细胞系M23分为HMGA1下调组、阴性对照组和空白组，分别转染HMGA1干扰序列、阴性对照序列和不作任何处理，采用实时荧光定量PCR术检测HMGA1 mRNA表达，CCK-8法检测细胞增殖能力，Transwell法检测细胞迁移和侵袭能力。

结果：UM组织中HMGA1蛋白阳性表达率为77%，高于正常葡萄膜组织中的29%(P<0.001)； 与未发生巩膜浸润、未累及睫状体和未发生眼外生长相比，HMGA1蛋白在发生巩膜浸润、累及睫状体和发生眼外生长的组织中阳性表达率升高(均P<0.05)。与阴性对照组和空白组相比，HMGA1 mRNA在HMGA1下调组细胞中相对表达量降低，且HMGA1下调组细胞培养24、48、72、96h时吸光度OD值降低，迁移细胞数和侵袭细胞数均明显减少(均P<0.05)。

结论：UM组织中HMGA1蛋白阳性表达率升高，下调M23细胞中HMGA1表达可减少细胞增殖，抑制细胞迁移和侵袭。

METHODS: A total of 53 cases(53 eyes)of UM patients who underwent surgical treatment in our hospital from February 2014 to August 2019 were selected. In the same period, 34 cases(34 eyes)of normal uveal tissues removed from the eye due to trauma were selected. The expression of HMGA1 protein in tissues was detected by immunohistochemistry. The human UM cell line M23 was cultured and divided into HMGA1 downregulation group, negative control group and blank group, respectively, transfected with HMGA1 interference sequence, negative control sequence and without any treatment. The expression of HMGA1 was detected by real-time quantitative PCR. The cell proliferation ability was detected by CCK-8 method, and the cell migration and invasion abilities were detected by Transwell method.

RESULTS: The positive expression rate of HMGA1 protein in UM tissue was 77%, which was higher than that in the normal uveal tissue, which was 29%(P<0.001). Compared with the no scleral occurring infiltration, no ciliary body involving, and no extraocular growth, the positive expression rates of HMGA1 proteins in the scleral infiltration, ciliary body involving, and extraocular growth occurring tissues were increased(all P<0.05). The relative expression level of HMGA1 mRNA in cells in the HMGA1 downregulation group was lower than that in the negative control group and the blank group. Compared with the negative control group and the blank group, the absorbance OD values of cells in the HMGA1 downregulation group at 24, 48, 72 and 96h were decreased(P<0.05). The number of migrating cells and the number of invading cells in the HMGA1 downregulation group was significantly less than those in the negative control group and the blank group(P<0.05).

CONCLUSION: The positive expression rate of HMGA1 protein in UM tissue is increased. Down regulation the expression of HMGA1 in M23 cells can reduce cell proliferation and inhibit cell migration and invasion.