目的：检测急性前葡萄膜炎(AAU)患者血清和房水中促红细胞生成素(EPO)、缺氧诱导因子-1α(HIF-1α)表达水平，探究二者的临床意义。方法：前瞻性研究。选取2018-01/2020-12本院收治的60例AAU患者为研究对象，同期增殖性玻璃体视网膜病变患者60例为对照组，收集两组患者血清及房水，使用酶联免疫吸附试验(ELISA)检测血清和房水中EPO、HIF-1α水平； 并对AAU患者进行焦虑自评量表(SAS)和抑郁自评量表(SDS)评价，疾病严重程度评分； Pearson法分析AAU患者SAS评分、SDS评分与血清和房水中EPO、HIF-1α水平的相关性； Spearman分析AAU患者疾病严重程度评分与血清和房水中EPO、HIF-1α水平的相关性。结果：与对照组相比，AAU患者血清和房水中EPO、HIF-1α水平均升高(P<0.01)。AAU患者SAS评分阴性23例、阳性37例，SDS评分阴性29例、阳性31例。与SAS评分阴性相比，SAS评分阳性血清中HIF-1α水平、房水中EPO水平升高(P<0.05)； 与SDS评分阴性相比，SDS评分阳性血清中EPO水平、房水中EPO、HIF-1α水平均升高(P<0.01)。与AAU轻度患者相比，重度患者血清和房水中EPO、HIF-1α水平均升高(P<0.01)。Pearson分析AAU患者SAS和SDS评分与血清和房水中EPO、HIF-1α水平无相关性(P>0.05)，血清和房水中EPO与HIF-1α水平均呈正相关(P<0.05)。Spearman分析AAU患者疾病严重程度评分与血清和房水中EPO、HIF-1α水平均呈正相关(P<0.05)。结论：AAU患者血清和房水中EPO、HIF-1α均高表达，且两者与疾病严重程度评分关系密切。

AIM: To detect the expression of erythropoietin(EPO)and hypoxia-inducible factor-1α(HIF-1α)in serum and aqueous humor of patients with acute anterior uveitis(AAU), and to explore their clinical significance. METHODS: From January 2018 to December 2020, 60 patients with AAU in our hospital were prospectively selected as the research objects, and 60 patients with proliferative vitreoretinopathy in the same period were taken as control(control group). The serum and aqueous humor of two groups were collected, enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of EPO and HIF-1α in serum and aqueous humor; the self-rating anxiety scale(SAS)and the self-rating depression scale(SDS)were used to evaluate the AAU patients, and the severity of the disease was scored; Pearson method was used to analyze the correlation between SAS score, SDS score and levels of EPO and HIF-1α in serum and aqueous humor, and the correlation between levels of EPO and HIF-1α in the serum and aqueous humor. Spearman was used to analyze the correlation between the disease severity score of AAU patients and the levels of EPO and HIF-1α in serum and aqueous humor. RESULTS: Compared with the control group, the levels of EPO and HIF-1α in the serum and aqueous humor of the study group were higher(P<0.01). Among AAU patients, 23 were negative of SAS score and 37 were positive, and 29 were negative of SDS score and 31 were positive. Compared with patients with negative SAS score, the level of HIF-1α in serum and the level of EPO in the aqueous humor were higher in patients with positive SAS score(P<0.05); compared with patients with negative SDS score, the level of EPO in serum and the levels of EPO and HIF-1α in aqueous humor were higher in patients with positive SDS score(P<0.01). There were 26 mild patients and 34 severe patients with AAU. Compared with mild patients with AAU, the levels of EPO and HIF-1α in serum and aqueous humor were increased in severe patients(P<0.01). Pearson analysis showed that the SAS and SDS scores of AAU patients were not significantly correlated with the levels of EPO and HIF-1α in serum and aqueous humor(P>0.05), there was a positive correlation between EPO and HIF-1α in serum(P<0.05), and between EPO and HIF-1α in aqueous humor(P<0.05). Spearman analysis showed that the disease severity score of AAU patients was positively correlated with the levels of EPO and HIF-1α in serum and aqueous humor(P<0.05). CONCLUSION: EPO and HIF-1α are highly expressed in serum and aqueous humor of AAU patients, and they are closely related. The two are closely related to the disease severity score, and should be paid attention to clinically.

湖南省临床医疗技术创新引导项目(No.2020SK50304)