目的：研究不同程度牵拉力对大鼠视网膜神经节细胞(RGCs)存活率和神经传导功能的影响，探讨RGCs自噬水平对上述指标的影响。

方法：选取健康雄性SD大鼠30只随机分为空白组、假手术组、0.15N组、0.3N组、0.6N组，每组各6只。模型组采用横向定量牵拉法制作视神经损伤大鼠模型。空白组大鼠不予处理。假手术组仅暴露视神经，不予牵拉。造模后第1、3d行闪光视觉诱发电位(f-VEP)检查，第3d取视网膜组织行Brn-3a免疫组织化学染色观察RGCs存活情况，透射电子显微镜观察自噬小体，蛋白质印迹法检测LC3BⅡ/Ⅰ蛋白表达水平。

结果：造模后第3d，与假手术组比较，模型组大鼠f-VEP P2潜伏期延长，振幅降低，视网膜组织中RGCs存活率降低，LC3BⅡ/Ⅰ蛋白表达水平降低，且各组大鼠视网膜组织中均可见自噬小体。

结论：视神经牵拉伤会降低大鼠早期视网膜自噬水平，导致RGCs死亡和相应的神经传导功能障碍，且不同牵拉力造成的损伤程度不同，RGCs存活情况可能与其自噬水平有关。

METHODS: A total of 30 healthy male Sprague-Dawley(SD)rats were randomly divided into empty group, sham-operation group, 0.15N, 0.3N and 0.6N group, with 6 rats in each group.Modeling group was performed the transverse quantitative traction to make a rat model of optic nerve injury. In addition, rats in empty group were not operated and rats in sham-operation group only got optic nerve exposed. Flash visual evoked potentials(f-VEP)were performed respectively on 1 and 3d after modeling.The survival of retinal ganglion cells was observed by Brn-3a staining at 3d after modeling, autophagy bodies were observed by transmission electron microscope, and the expression level of LC3B II/I protein was detected by Western blotting.

RESULTS: Compared with sham-operation group, the f-VEP P2 peak was significantly delayed and the amplitude reduced at 3d after modeling. In addition, the survival rate of RGCs was decreased, and the expression level of LC3B II/I protein were decreased. Autophagy bodies were observed in the retinal tissue of rats in all groups.

CONCLUSION: Optic nerve traction reduced early retinal autophagy level, death of RGCs and corresponding nerve conduction dysfunction in rats,and different traction caused different degrees of injury. In addition,there was a correlation between the autophagy level and the survival of RGCs.