目的：探索小鼠视网膜色素变性(RP)模型视网膜内质网应激免疫组织化学(IHC)染色抗体的最适浓度，为研究RP发病机制及干预措施提供相应的指标检测方法。

方法：给予清洁级健康雄性C57BL/6J小鼠腹腔注射N-甲基-N-亚硝基脲(MNU，60mg/kg)制备RP小鼠模型，造模后第7d行视网膜电图(ERG)检测和苏木精-伊红(HE)染色验证造模成功，摘取眼球并采用IHC染色法检测视网膜内质网应激相关蛋白(IRE1、ATF6、PERK、GRP78、Caspase-12)表达情况。

结果：IRE1、ATF6、PERK、GRP78、Caspase-12蛋白均在RP小鼠视网膜呈阳性表达，IRE1抗体浓度为1：1000、ATF6抗体浓度为1：500和1：1000(此抗体两种浓度阳性表达无差异，P>0.05)、PERK抗体浓度为1：1500、GRP78抗体浓度为1：200、Caspase-12抗体浓度为1：100时，阳性表达强度适宜，且与各自其余抗体浓度相应蛋白阳性表达均有差异(P<0.05)。

结论：内质网应激相关蛋白抗体浓度IRE1为1：1000、ATF6为1：500和1：1000、PERK为1：1500、GRP78为1：200、Caspase-12为1：100时，其在小鼠RP模型视网膜IHC染色阳性表达程度较适宜。

METHODS: Clean male C57BL/6J mice were intraperitoneally injected with N-methyl-N-nitrosourea(MNU, 60mg/kg)to prepare RP mouse model. Electroretinogram(ERG)and hematoxylin-eosin(HE)staining were performed on 7d after modeling to verify the successful modeling. The expression of endoplasmic reticulum stress-related proteins(IRE1, ATF6, PERK, GRP78, Caspase-12)was detected by IHC staining.

RESULTS: The following proteins, including IRE1, ATF6, PERK, GRP78 and Caspase-12, were positively expressed in retina of RP mouse. The optimal concentrations of the above proteins were as follows: IRE1 antibody concentration was 1:1000, ATF6 antibody concentration was 1:500 and 1:1000(with no difference in positive expression, P>0.05), PERK antibody concentration was 1:1500, GRP78 antibody concentration was 1:200 and Caspase-12 antibody concentration was 1:100, the proteins were well expressed at the above concentrations, and the positive expressions of corresponding proteins were different from those of other antibody concentrations(P<0.05).

CONCLUSION: The optimal concentrations for IHC staining in different proteins of mouse RP models were as follows: the concentrations of endoplasmic reticulum stress-related protein antibodies were 1:1000 in IRE1, 1:500 and 1:1000 in ATF6, 1:1500 in PERK, 1:200 in GRP78, and 1:100 in Caspase-12.