目的：探讨miR-375表达对脉络膜黑色素瘤MUM-2B细胞增殖和侵袭的影响。

方法：培养MUM-2B细胞，分别转染miR-375模拟物序列(模拟物组)、miR-375抑制物序列(抑制物组)、阴性对照序列(阴性对照组)和不做任何处理(空白组)，分别采用qRT-PCR实验、CCK-8实验、细胞凋亡实验、Transwell实验检测细胞中miR-375、细胞增殖活性、细胞凋亡情况、细胞迁移和侵袭情况。

结果：相比于阴性对照组(1.01±0.10)和空白组(1.03±0.07)，模拟物组细胞中miR-375表达量(2.65±0.15)升高，而抑制物组细胞中miR-375表达量(0.28±0.06)降低(P<0.05)； 与空白组和阴性对照组比较，模拟物组细胞24、48、72和96h时OD值均降低(P<0.05)，而抑制物组细胞24、48、72和96h时OD值均升高(P<0.05)； 与空白组细胞凋亡率(20.54±4.01)%和阴性对照组细胞凋亡率(22.80±4.28)%比较，模拟物组细胞凋亡率(39.11±3.37)%升高(P<0.05)，而抑制物组细胞凋亡率(10.13±2.17)%降低(P<0.05)； 与空白组和阴性对照组比较，模拟物组细胞迁移数和细胞侵袭数均降低(P<0.05)，而抑制物组细胞迁移数和细胞侵袭数均升高(P<0.05)。

结论：上调MUM-2B细胞中miR-375表达可降低细胞增殖活性，加速细胞凋亡，抑制细胞迁移和侵袭，下调miR-375表达则发挥相反的作用，表明miR-375可能在脉胳膜黑色素瘤病程中发挥抑癌功能。

METHODS: MUM-2B cells were cultured and were transfected with miR-375 mimic sequence(mimic group), miR-375 inhibitor sequence(inhibitor group), negative control group and no treatment(blank group). The qRT-PCR, CCK-8, apoptosis and Transwell experiments were used respectively to detect the expression of miR-375, cell proliferation activity, apoptosis, cell migration and invasion.

RESULTS: Compared with the negative control group(1.01±0.10)and the blank group(1.03±0.07), the expression level of miR-375 in the cells of the mimic group(2.65±0.15)was increased, while the expression level of miR-375 in the cells of the inhibitor group(0.28±0.06)was decreased(P<0.05). Compared with the blank group and negative control group, the OD values of the cells in the mimic group at 24, 48, 72, and 96h were decreased(P<0.05), while the OD values of the cells in the inhibitor group at 24, 48, 72, and 96h were increased(P<0.05). Compared with the apoptosis rates in the blank group and negative control group, which were(20.54±4.01)% and(22.80±4.28)%, the apoptosis rate in the mimic group(39.11±3.37)% was increased(P<0.05), while it was decreased in the inhibitor group(10.13±2.17)%(P<0.05). Compared with the blank group and negative control group, the number of migration cell and the number of invasion cell in the mimic group were decreased(P<0.05), while the number of migration cell and the number of invasion cell in the inhibitor group were increased(P<0.05).

CONCLUSIONS: Up-regulating the expression of miR-375 in MUM-2B cells can reduce cell proliferation activity, accelerate cell apoptosis, and inhibit cell migration and invasion, while down-regulating the expression of miR-375 has the opposite effect. It indicates that miR-375 may play the function of tumor suppressor in the course of CM.