干眼是指由泪液的质、量和动力学异常等原因导致的泪膜稳定性下降或眼表微环境失衡，造成多种眼部不适甚至视功能障碍。其发病机制复杂，目前治疗尚局限于缓解症状，保护视功能。针刺治疗干眼有效，但作用机制尚未完全明确。蛋白质组学技术能系统全面反映蛋白质的功能、结构以及相互作用关系，将蛋白质组学技术应用于针刺治疗干眼的研究，不仅可从不同病因病程揭示干眼蛋白质水平的动态变化，寻找潜在生物标志物，还能系统挖掘针刺治疗干眼的调控机制，为针刺治疗的理论研究和针刺作用的基础研究提供依据。本文旨在探讨蛋白质组学在干眼临床和基础研究中的潜在应用价值，为干眼的诊疗及针刺机制研究提供科学有效的思路。

Dry eye disease(DED)refers to a condition characterized by reduced stability of the tear film or an imbalance in the microenvironment of the ocular surface, resulting from abnormalities in quality, quantity and kinetics of tear. This condition leads to various ocular discomforts and even visual impairment. The pathogenesis of DED is multifactorial and current treatment mainly focuses on symptom relief and preservation of visual function. Acupuncture has shown effectiveness in treating dry eye, although its underlying mechanism remains incompletely understood. Proteomics technology offers a comprehensive and systematic approach to studying the functions, structures and interactions of proteins. Its application in DED research can provide valuable insights into the dynamic changes in protein levels associated with different etiology or the course of DED and facilitate the identification of potential biomarkers. Furthermore, proteomics can systematically explore the regulatory mechanisms underlying acupuncture treatment for DED, providing a theoretical basis for acupuncture treatment research and contributing to the understanding of its effects at a fundamental level. This paper aims to explore the potential application of proteomics in both clinical and basic research on DED. Ultimately, it strives to offer scientific and effective strategies for the diagnosis and treatment of DED and advance our knowledge of the mechanisms underlying acupuncture therapy.

国家自然科学基金面上项目(No.81774419); 国家自然科学基金青年项目(No.82205259); 江苏省卫生健康发展研究中心开放课题(No. JSHD2021053); 南京市中医药科技专项项目(No. ZYYB202229)