Home > Archive>Volume 13, Issue 4, 2020 >545-551. DOI:10.18240/ijo.2020.04.03
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Involvement of moesin phosphorylation in ischemia/reperfusion induced inner blood-retinal barrier dysfunction
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Corresponding Author:

Jing Wu. Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China. wujingsci@126.com

Fund Project:

Supported by the Science and Technology Planning Project of Guangdong Province (No.201607010386); the Science and Technology Planning Project of Guangzhou (No.201504290959196).

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    Abstract:

    AIM: To investigate the role of moesin and its underlying signal transduction in retinal vascular damage induced by retinal ischemia-reperfusion (RIR) insult. METHODS: C57BL/6 mice were subjected to continued ischemia for 45min, followed by blood reperfusion. The expression and phosphorylation of moesin in retinal vessels were detected by immunohistochemistry and Western blotting. The inner blood-retinal barrier was evaluated using FITC-dextran leakage assay on whole-mount retina. Further studies were conducted to explore the effects of p38 mitogen-activated protein kinase (MAPK) pathway on the involvement of moesin in RIR-evoked retinal vascular hyperpermeability response. RESULTS: It revealed that RIR induced moesin phosphorylation in a time-dependent manner after reperfusion. The phosphorylation of moesin was alleviated by inhibitions of p38 MAPK, while this treatment also ameliorated the dysfunction of inner blood-retinal barrier. CONCLUSION: The results suggest that moesin is involved in RIR-evoked retinal vascular endothelial dysfunction and the phosphorylation of moesin is triggered via p38 MAPK activation.

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Jing Xu, Qiong Liu, Ming Ma,/et al.Involvement of moesin phosphorylation in ischemia/reperfusion induced inner blood-retinal barrier dysfunction. Int J Ophthalmol, 2020,13(4):545-551

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Publication History
  • Received:August 29,2019
  • Revised:February 12,2020
  • Online: March 03,2020
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