AIM: To observe the effect of different concentrations Buddleja offcinalis eye drops (BOED) to inflammatory factors of lacrimal gland cells of castrated male rabbit with dry eye. METHODS: Forty-two healthy adult New Zealand rabbits were randomly divided into 7 groups, 6 rabbits each group. Group A: blank group, Group B: model group, Group C: low concentrations BOED group, Group D: medium concentrations BOED, Group E: high concentrations BOED, Group F: the placebo group, Group G: testosterone groups. All rabbits of group B to G were cut off bilateral testis and epididymis except group A. The rabbits of group C, D, E and F were dropped relevant eye drops except groups A and B. All rabbits were tested Schimer I test (SIT) and break-up time (BUT) before operation and 4wk after operation. Expressions of inflammatory factors of TNF-α, IL-1β from lacrimal gland cells were checked in immunohistochemical staining method. RESULTS: 1) Comparison of SIT and BUT: Compared with the groups D, E and G, group C had statistical significance (P<0.01). Compared with group D and E, G groups, there was no significant difference among those group before and after operation (P>0.05); 2) Comparison of inflammatory factors of TNF-α, IL-1β after operation: Compared with value of the average optical density of TNF-α and IL-1β of group C, those of group D, E and G were obviously different after operation (P<0.05). Compared D and with E, G groups, there was no significant difference between those group after operation (P>0.05).