AIM: To determine the status of non-strabismic binocular vision anomalies (NSBVA) among students of a Malaysian private university uses visual display units (VDU). METHODS: A cross-sectional study was conducted among university students who use VDU 3h or more from January 2019 to May 2019. A convenient sampling method was utilized. All subjects had gone through primary eye-examinations to satisfy the inclusion criteria. Those who satisfy the inclusion criteria, further gone through the NSBVA assessment. The descriptive analysis was done to rule out the percentage of NSBVA and Chi-square test of independence was carried out to observe the association of NSBVA with age, gender and hours of VDU usage. RESULTS: A total of 140 students including 88 females (62.9%) and 52 males (37.1%) participated in this study. The mean age of the participants was 22.54±1.48 years and the mean VDU usage hours were 5.76±2.49h. The percentage of NSBVA is 40% among the students those who use VDU. The occurrence of accommodative and vergence anomalies among the VDU users is 17.86% and 22.14% respectively. There was a moderate association between gender and NSBVA (P=0.010). However, there was no significant association observed for age (P=0.334) and hours of VDU usage (P=0.835) with NSBVA. CONCLUSION: NSBVA is 40% among the students of a Malaysian private university uses VDU. Accommodation insufficiency (15%) and convergence insufficiency (10%) is more common among all NSBVA for VDU users.